Atcc hl-60

THE NCI-60 PANEL. Cell lines with an ATCC number. DNA. Protein. ref. consensus. comment HL-60, CCL-240, R248L, 1018. p53 null. null, p.M1_* 394del

96100920 . Human promyelocytic leukaemic cells established from HL60 cells by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR . July 2018 HL-60 (ATCC® CCL-240™). HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, HL-60 (ATCC CCL-240). ATCC® Number: CCL-240. Organism: Homo sapiens, human.

21.02.2021 Atcc hl-60

However, 1,25-(OH)2D3 inhibited the proliferation of LG cells to the same degree as that … When unopsonized, Wolinella recta ATCC 33228 significantly suppressed the net proliferation of uninduced HL-60 cells, Actinobacillus actinomycetemcomitans strain Y4 was markedly lethal to the cells, and Bacteroides gingivalis ATCC 33277 had no effect. Unopsonized and opsonized A. actinomycetemcomitans and W. recta had equally potent lethal effects on induced HL-60 cells. … I got a HL-60 cells from ATCC and cultured them in IMEM high glucose+20% FBS (non heat inactivated as recommend by atcc technical support)and cryopreserved the cells at a density if 4*105 cells/ml. Eos-HL-60 . 96100920 . Human promyelocytic leukaemic cells established from HL60 cells by sub-cloning in methylcellulose.

Clone, HL-60, HL60 Human caucasian promyelocytic leukaemia The clone 15 HL-60 has been derived from HL-60 cells (ECACC catalogue no. 98070106) that had been cultured at an elevated pH of 7.6 - 7.8 for 2 months. Clone 15 HL-60 was picked from soft agar being selected for the ability to differentiate to eosinophils after treatment with butyric acid.

The Cellosaurus identifier is a combination of the cell line name variant chosen by Cellosaurus, with the Cellosaurus accession ID, the latter written as RRID. > ATCC > hl60 cells. hl60 cells ATCC is a verified supplier ATCC manufactures this product .

Mar 24, 2005 · HL‐60 cell culture, differentiation and labelling. The HL‐60 cell line was obtained from ATCC and maintained in Iscove's modified Dulbecco's medium (Invitrogen, Grand Island, NY) supplemented with 10% fetal bovine serum, 50 U/ml penicillin, and 50 µg/ml streptomycin at 37° with 5% CO 2.

Disease: Acute SAFETY PRECAUTION. Unpacking & Storage Instructions. Handling Procedure for Frozen Cells. Page 1 of 3. Product Sheet.

Applications: This cell line is a suitable transfection … 17/02/2020 Page 3 of 3 3URGXFW6KHHW HL60/MX1 (ATCC® CRL 2258™) Please read this FIRST 6WRUDJH7HPS liquid nitrogen vapor phaae %LRVDIHW\/HYHO 1 Intended Use The human promyelocytic leukemia cell line, HL-60 (CCL24O;ATCC, Rockville, MD) was cultured in plastic dishes at 37Â°Cin RPMI1640 (Nissui, Tokyo, Japan)supplementedwith 10%fetal calf serum (Irvine Scientific,Santa Ana, CA) and 100p@g/mlkanamycin.The HL-60 cells used in this work were restricted to low passages characterized by a doubling time of 47 h. Three high passage HL-60 subclones, isolated … DNA into HL-60 Human lymphoblasts (ATCC Cat. No. CCL-240) using Lipofectamine To transfect HL-60 cells in different tissue culture formats, vary the amounts of Lipofectamine ™ LTX Reagent, DNA, cells, medium and PLUS™ Reagent used in proportion to the relative surface area, as shown in the table (amounts given on a per well basis).

Characteristics. Derivation. HL-60/MX1 is a mitoxantrone resistant derivative of the HL-60 cell line (see ATCC CCL-240) which was obtained from peripheral blood Clone 15 HL-60 (ATCC® CRL-1964™). This clone, picked from soft agar, was selected for its ability to undergo eosinophilic differentiation when treated with DNA purified from ATCC CCL-240, HL-60, acute promyelocytic leukemia cell line . MoreLess.

Techniques: Incubation, Plasmid Preparation. Journal: Infection and Immunity. Article Title: Actinobacillus actinomycetemcomitans Leukotoxin Induces Apoptosis in HL-60 Cells. doi: Figure Lengend Snippet: … HL-60 from ATCC. Description HL-60 is a promyelocytic cell line derived by S.J. Collins, et al.

Oct 01, 2012 · HL-60 cells were obtained from the American Type Culture Collection (ATCC) and cultured in IMDM medium with 10% fetal bovine serum (FBS) at 37°C in a fully humidified atmosphere with 5%CO 2. Half of fresh medium were replaced every other day. The protocols I have read seem straightforward on differentiating HL-60 cells, however I am not having luck using 1.3% DMSO and/or 1uM ATRA (all trans retinoic acid). I culture in 1640 RPMI Apr 15, 2010 · HL-60 cells (CCL-240) and U-937 cells (CRL-1593.2) were supplied by American Type Culture Collection (ATCC). HL-60 cultures were maintained at concentrations between 1×10 5 and 1×10 6 of viable cells/ml at 37°C under 5% CO 2 in air and 95% humidity, in Iscove's modified Dulbecco's medium (IMDM) supplemented with 20% heat-inactivated fetal Transfection of HL-60 Cells Use this procedure to transfect plasmid DNA into HL-60 cells in a 24-well format (for other formats, see Scaling Up or Down Transfections, below).

Half of fresh medium were replaced every other day. The protocols I have read seem straightforward on differentiating HL-60 cells, however I am not having luck using 1.3% DMSO and/or 1uM ATRA (all trans retinoic acid). I culture in 1640 RPMI Apr 15, 2010 · HL-60 cells (CCL-240) and U-937 cells (CRL-1593.2) were supplied by American Type Culture Collection (ATCC). HL-60 cultures were maintained at concentrations between 1×10 5 and 1×10 6 of viable cells/ml at 37°C under 5% CO 2 in air and 95% humidity, in Iscove's modified Dulbecco's medium (IMDM) supplemented with 20% heat-inactivated fetal Transfection of HL-60 Cells Use this procedure to transfect plasmid DNA into HL-60 cells in a 24-well format (for other formats, see Scaling Up or Down Transfections, below).

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HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia. To ATCC Valued Customers,

(f – i) Enlarged images of cells during separation from Figure 2 e. HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e.

HL-60/MX1 (see ATCC CRL-2258) cells were selected and subcloned in 1987 for resistance to 39 nM mitoxantrone, an anthracenedione antitumor agent. Subsequent exposure of the HL-60/MX1 cells to higher concentrations of mitoxantrone led to the emergence of cells capable of growth at a concentration of 190 nM.

The ATCC Genome Portal makes it easy to find the high-quality whole-genome sequencing data needed for your research. With a free account, you can easily search, download, and analyze hundreds of high-quality genomes. HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT.

I cryopreserved HL-60 cells in FBS/ 10% DMSO but unfortunately cannot recover these cells properly. The cells have a good appearance at day 1 and 2. However, significantly lower cells counts are HL-60 and KG1 cells were treated for 16 h with 5 µM DDA or 0.1 µM Ara-C, alone or in combination. (A) The presence of acidic vesicles was revealed using Cyto-ID.